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Article Dans Une Revue Gene Année : 1994

Direct PCR sequencing of the ndd gene of bacteriophage T4: identification of a product involved in bacterial nucleoid disruption

Résumé

The rapid disruption of the Escherichia coli nucleoid after T4 infection requires the activity of the phage-encoded ndd gene. We have genetically identified the sequence encoding ndd. Determination of the sequence of a 2.5-kb segment including ndd closed the last significant gap in the sequence of the T4 genome. This analysis was performed on PCR-amplified fragments that were purified by gel-exclusion chromatography and then submitted to linear amplification cycle sequencing. This technology permitted sequence comparison of two ndd mutants (ndd44 and ndd98) with the wild-type gene. The analysis of ndd from six bacteriophages of the T-even family indicated that the protein encoded by this nonessential gene is surprisingly conserved.

Dates et versions

hal-02713863 , version 1 (01-06-2020)

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Jean-Yves Bouet, J. Woszczyk, Francis Repoila, V. François, J. M. Louarn, et al.. Direct PCR sequencing of the ndd gene of bacteriophage T4: identification of a product involved in bacterial nucleoid disruption: Sequencing of PCR fragments; insertional mutagenesis; analysis of toxic genes. Gene, 1994, 141 (1), pp.9-16. ⟨10.1016/0378-1119(94)90121-X⟩. ⟨hal-02713863⟩

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