NMR metabolomics for understanding the role of alkaline phosphatase in neurotransmission and inflammation processes - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Poster De Conférence Année : 2020

NMR metabolomics for understanding the role of alkaline phosphatase in neurotransmission and inflammation processes

Résumé

Introduction Tissue Non-specific Alkaline Phosphatase (TNAP) is a ubiquitous ectoenzyme located in different body tissues. Mutations of the TNAP gene are responsible for hypophosphatasia, a rare disease characterized mainly by defective bone mineralization caused by pyrophosphate accumulation in bone tissue and accompanied by neurological symptoms (pain, seizure…) [1]. We aimed in identifying metabolic pathways associated with TNAP activity in brain and in exploring its role in the inflammatory process.Technological and methodological innovation Untargeted 1H NMR-based metabolomics was used on tissue extracts from two original models: (i) Akp2 mice, which is the rodent model of hypophosphatasia by invalidation of the TNAP gene and (ii) a model of postprandial inflammation based on food intake [2]. Results and impact Eight metabolites differentiated TNAP-deficient mouse brain (in 7 day-old animals), among which GABA, adenosine and cystathionine were the most discriminant [3]. Metabolic changes were also observed in both liver and brain in the postprandial inflammation model. These metabolic changes suggest that TNAP is (i) involved in the production of anti-inflammatory agents (adenosine) or their precursor (cystathionine, precursor of H2S), (ii) regulated by pro-inflammatory cytokines

Domaines

Chimie
Fichier principal
Vignette du fichier
Poster_Hypo_def.pdf (1.13 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02875612 , version 1 (19-08-2022)

Identifiants

  • HAL Id : hal-02875612 , version 1

Citer

Stéphane Balayssac, Thomas Cruz, Marie Gleizes, Gary Lutin, Juana Como, et al.. NMR metabolomics for understanding the role of alkaline phosphatase in neurotransmission and inflammation processes. European RFMF Metabomeeting, Jan 2020, Toulouse, France. ⟨hal-02875612⟩
96 Consultations
18 Téléchargements

Partager

Gmail Facebook X LinkedIn More