Promotion through external magnetic field of osteogenic differentiation potential in adipose‐derived mesenchymal stem cells: Design of polyurethane/poly(lactic) acid sponges doped with iron oxide nanoparticles - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biomedical Materials Research Part B: Applied Biomaterials Année : 2020

Promotion through external magnetic field of osteogenic differentiation potential in adipose‐derived mesenchymal stem cells: Design of polyurethane/poly(lactic) acid sponges doped with iron oxide nanoparticles

Résumé

Recently, iron oxide nanoparticles (IONPs) have gathered special attention in regenerative medicine. Owing to their magnetic and bioactive properties, IONPs are utilized in the fabrication of novel biomaterials. Yet, there was no report regarding thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) polymer doped with IONPs on osteogenic differentiation of mesenchymal stem cells. Thus the objectives of presented study was to: (a) fabricate magnetic TPU + PLA sponges doped with iron (III) oxide Fe2O3 nanoparticles; (b) investigate the effects of biomaterial and its exposition to static magnetic field (MF) on osteogenic differentiation, proliferation, and apoptosis in adipose‐derived mesenchymal stem cells (ASCs). TPU + PLA sponges were prepared using solvent casting technique while incorporation of the Fe2O3 nanoparticles was performed with solution cast method. RT‐PCR was applied to evaluate expression of osteogenic‐related genes and integrin's in cells cultured on fabricated materials with or without the stimulation of static MF. MF stimulation enhanced the expression of osteopontin and collagen type I while decreased expression of bone morphogenetic protein 2 in tested magnetic materials—TPU + PLA/1% Fe2O3 and TPU + PLA/5% Fe2O3. Therefore, TPU + PLA sponges doped with IONPs and exposure to MF resulted in improved osteogenic differentiation of ASC.

Domaines

Chimie Matériaux
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Dates et versions

hal-02879241 , version 1 (23-06-2020)

Identifiants

Citer

K. Marycz, M. Alicka, K. Kornicka-Garbowska, J. Polnar, A. Lis-Bartos, et al.. Promotion through external magnetic field of osteogenic differentiation potential in adipose‐derived mesenchymal stem cells: Design of polyurethane/poly(lactic) acid sponges doped with iron oxide nanoparticles. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2020, 108, pp.1398-1411. ⟨10.1002/jbm.b.34488⟩. ⟨hal-02879241⟩
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