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Chapitre D'ouvrage Année : 2020

Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More

Michael Ryckelynck

Résumé

The central role of RNA in living systems made it highly desirable to have non-invasive and sensitive technologies allowing for imaging the synthesis and the location of these molecules in living cells. This need motivated the development of small pro-e ce ec e ca ed e a bec e e ce b d e e ca e c dab e RNA ca ed-a a e. Ye , e development of these fluorogen/light-up RNA pairs is a long and thorough process starting with the careful design of the fluorogen and pursued by the selection of a specific and efficient synthetic aptamer. This chapter summarizes the main design and the selection strategies used up to now prior to introducing the main pairs. Then, the vast application potential of these molecules for live-cell RNA imaging and other applications will be presented and discussed. Running title: Light-up RNA aptamers
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Dates et versions

hal-02953767 , version 1 (30-09-2020)

Identifiants

Citer

Michael Ryckelynck. Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More. RNA Tagging Methods and Protocols, pp.73-102, 2020, Methods in molecular biology, ⟨10.1007/978-1-0716-0712-1_5⟩. ⟨hal-02953767⟩

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