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Article Dans Une Revue EMBO Reports Année : 2020

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis

Nicolas Taulet
  • Fonction : Auteur
Audrey Guesdon
  • Fonction : Auteur
Audrey Douanier
  • Fonction : Auteur
Aurelie Dosdane
  • Fonction : Auteur
Melanie Cisneros
  • Fonction : Auteur
Justine Maurin
  • Fonction : Auteur
Sabrina Hettinger
  • Fonction : Auteur
Christelle Anguille
  • Fonction : Auteur
Michael Taschner
  • Fonction : Auteur
Esben Lorentzen
  • Fonction : Auteur
Benedicte Delaval

Résumé

Centrosome amplification is a hallmark of cancer, and centrosome clustering is essential for cancer cell survival. The mitotic kinesin HSET is an essential contributor to this process. Recent studies have highlighted novel functions for intraflagellar transport (IFT) proteins in regulating motors and mitotic processes. Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET are required for efficient centrosome clustering. We identify a direct interaction between the kinesin HSET and IFT proteins, and we define how IFT proteins contribute to clustering dynamics during mitosis using high-resolution live imaging of centrosomes. Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation. Overall, our data unravel a novel role for the IFT machinery in centrosome clustering during mitosis in cells harboring supernumerary centrosomes.
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Origine : Publication financée par une institution

Dates et versions

hal-02989234 , version 1 (25-11-2020)

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Benjamin Vitre, Nicolas Taulet, Audrey Guesdon, Audrey Douanier, Aurelie Dosdane, et al.. IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. EMBO Reports, 2020, 21 (6), ⟨10.15252/embr.201949234⟩. ⟨hal-02989234⟩
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