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Chapitre D'ouvrage Année : 2019

Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization

Résumé

The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes .
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Dates et versions

hal-03004884 , version 1 (13-11-2020)

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Brice Moukengue, Jerome Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, et al.. Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization. Methods in molecular biology, 1914, pp.169-196, 2019, ⟨10.1007/978-1-4939-8997-3_9⟩. ⟨hal-03004884⟩
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