Transketolase of Staphylococcus aureus in the Control of Master Regulators of Stress Response During Infection - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Infectious Diseases Année : 2019

Transketolase of Staphylococcus aureus in the Control of Master Regulators of Stress Response During Infection

Elodie Ramond
Anne Jamet
Jean-Philippe Barnier
Daniel Euphrasie
  • Fonction : Auteur
  • PersonId : 913259
Ivan Nemazanyy
Xavier Nassif
Alain Charbit
Mathieu Coureuil

Résumé

Staphylococcus aureus is a leading cause of both acute and chronic infections in humans. The importance of the pentose phosphate pathway (PPP) during S. aureus infection is currently largely unexplored. In the current study, we focused on one key PPP enzyme, transketolase (TKT). We showed that inactivation of the unique gene encoding TKT activity in S. aureus USA300 (∆tkt) led to drastic metabolomic changes. Using time-lapse video imaging and mice infection, we observed a major defect of the ∆tkt strain compared with wild-type strain in early intracellular proliferation and in the ability to colonize kidneys. Transcriptional activity of the 2 master regulators sigma B and RpiRc was drastically reduced in the ∆tkt mutant during host cells invasion. The concomitant increased RNAIII transcription suggests that TKT-or a functional PPP-strongly influences the ability of S. aureus to proliferate within host cells by modulating key transcriptional regulators.
Fichier principal
Vignette du fichier
Tan et al JID authors version.pdf (7.48 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-03012156 , version 1 (18-11-2020)

Identifiants

Citer

Xin Tan, Elodie Ramond, Anne Jamet, Jean-Philippe Barnier, Baptiste Decaux-Tramoni, et al.. Transketolase of Staphylococcus aureus in the Control of Master Regulators of Stress Response During Infection. Journal of Infectious Diseases, 2019, 220 (12), pp.1967 - 1976. ⟨10.1093/infdis/jiz404⟩. ⟨hal-03012156⟩
39 Consultations
47 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More