Extracellular vesicles of MSCs and cardiomyoblasts are vehicles for lipid mediators - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Biochimie Année : 2020

Extracellular vesicles of MSCs and cardiomyoblasts are vehicles for lipid mediators

Résumé

Recent works reported the relevance of cellular exosomes in the evolution of different pathologies. However, most of these studies focused on the ability of exosomes to convey mi-RNA from cell to cell. The level of knowledge concerning the transport of lipid mediators by these nanovesicles is more than fragmented. The role of lipid mediators in the inflammatory signaling is fairly well described, in particular concerning the derivatives of the arachidonic acid (AA), called eicosanoïds or lipid mediators. The aim of the present work was to study the transport of these lipids within the extracellular vesicles of rat bone marrow mesenchymal stem cells (BM-MSC) and the cardiomyoblast cell line H9c2. We were able to characterize, for the first time, complete profiles of oxilipins within these nanovesicles. We studied also the impact on these profiles, of the polyunsaturated fatty acids (PUFAs) know to be precursors of the inflammatory signaling molecules (AA, eicosapentaenoic acid EPA and Docosahexaenoic acid DHA), at physiological concentrations. By growing the progenitor cells under PUFAs supplementation, we provide a comprehensive assessment of the beneficial effect of u-3 PUFA therapy. Actually, our results tend to support the resolving role of the inflammation that stromal cell-derived extracellular vesicles can have within the cardiac microenvironment.
Fichier principal
Vignette du fichier
biochimie- EV signalization author manuscrit (1).pdf (1.03 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03018214 , version 1 (30-11-2020)

Identifiants

Citer

Nathalie Pizzinat, Varravaddheay Ong-Meang, Florence Bourgailh-Tortosa, Muriel Blanzat, Lucie Perquis, et al.. Extracellular vesicles of MSCs and cardiomyoblasts are vehicles for lipid mediators. Biochimie, 2020, 178, pp.69-80. ⟨10.1016/j.biochi.2020.07.013⟩. ⟨hal-03018214⟩
45 Consultations
246 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More