%0 Journal Article %T Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases %+ Nanomédecine Régénérative (NanoRegMed) %+ Service d’Hypertension et Maladies Vasculaires %A Gaertner, Sébastien %A Auger, Cyril %A Farooq, Muhammad, A %A Pollet, Brigitte %A Khemais-Benkhiat, Sonia %A Niazi, Zahid, R %A Schrevens, Sophie %A Park, Sin-Hee %A Toti, Florence %A Stephan, Dominique %A Schini-Kerth, Valérie, B %< avec comité de lecture %@ 1661-6596 %J International Journal of Molecular Sciences %I MDPI %V 21 %P 113749 %8 2020-01-30 %D 2020 %R 10.3390/ijms21030920 %K endothelial dysfunction %K aging %K omega-3 polyunsaturated fatty acids %K cyclooxygenases %K venous thrombosis %Z Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system %Z Life Sciences [q-bio]/Pharmaceutical sciences/PharmacologyJournal articles %X In humans, aging is associated with endothelial dysfunction and an increased risk of venous thromboembolism. Although intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at a ratio of 6:1 by old rats improved the endothelial dysfunction in arteries, the impact on veins remains unclear. Eight-month-old male Wistar rats were either untreated or orally administered corn oil, EPA:DHA 1:1, or EPA:DHA 6:1 (500 mg/kg/d) for seven days. Vascular reactivity was studied by myography. In middle-aged femoral artery rings, acetylcholine caused a partial relaxation at low concentrations and a contractile response at high concentrations, whereas in the old femoral vein only a partial relaxation was observed. The EPA:DHA 6:1 treatment blunted the contractile response to acetylcholine in the middle-aged femoral artery and both EPA:DHA 6:1 and 1:1 increased the relaxation to acetylcholine in the old femoral vein. No such effects were observed with corn oil. Both the non-selective cyclooxygenase inhibitor indomethacin and the COX-1 inhibitor SC-560 increased the relaxation to acetylcholine in the middle-aged femoral artery whereas the COX-2 inhibitor NS-398 increased that in the middle-aged femoral vein. In conclusion, our results indicate that aging is associated with an endothelial dysfunction in the femoral artery and vein, which can be improved by EPA:DHA 6:1 treatment-most likely via a cyclooxygenase-dependent mechanism. %G English %2 https://cnrs.hal.science/hal-03033691/document %2 https://cnrs.hal.science/hal-03033691/file/Gaertner-2020-Oral-intake-of-epadha--by-middle-ag.pdf %L hal-03033691 %U https://cnrs.hal.science/hal-03033691 %~ INSERM %~ CNRS %~ CARDIO %~ UNIV-STRASBG %~ SITE-ALSACE