Parabolic, Flight-Induced, Acute Hypergravity and Microgravity Effects on the Beating Rate of Human Cardiomyocytes - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cells Année : 2019

Parabolic, Flight-Induced, Acute Hypergravity and Microgravity Effects on the Beating Rate of Human Cardiomyocytes

Aviseka Acharya
  • Fonction : Auteur
  • PersonId : 1084233
Sonja Brungs
  • Fonction : Auteur
  • PersonId : 1084234
Yannick Lichterfeld
  • Fonction : Auteur
  • PersonId : 1084235
Jürgen Hescheler
  • Fonction : Auteur
  • PersonId : 1084236
Ruth Hemmersbach
  • Fonction : Auteur
  • PersonId : 1084237
Agapios Sachinidis
  • Fonction : Auteur
  • PersonId : 1084238

Résumé

Functional studies of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hCMs) under different gravity conditions contribute to aerospace medical research. To study the effects of altered gravity on hCMs, we exposed them to acute hypergravity and microgravity phases in the presence and absence of the β-adrenoceptor isoprenalin (ISO), L-type Ca 2+ channel (LTCC) agonist Bay-K8644, or LTCC blocker nifedipine, and monitored their beating rate (BR). These logistically demanding experiments were executed during the 66th Parabolic Flight Campaign of the European Space Agency. The hCM cultures were exposed to 31 alternating hypergravity, microgravity, and hypergravity phases, each lasting 20-22 s. During the parabolic flight experiment, BR and cell viability were monitored using the xCELLigence real-time cell analyzer Cardio Instrument ®. Corresponding experiments were performed on the ground (1 g), using an identical setup. Our results showed that BR continuously increased during the parabolic flight, reaching a 40% maximal increase after 15 parabolas, compared with the pre-parabolic (1 g) phase. However, in the presence of the LTCC blocker nifedipine, no change in BR was observed, even after 31 parabolas. We surmise that the parabola-mediated increase in BR was induced by the LTCC blocker. Moreover, the increase in BR induced by ISO and Bay-K8644 during the pre-parabola phase was further elevated by 20% after 25 parabolas. This additional effect reflects the positive impact of the parabolas in the absence of both agonists. Our study suggests that acute alterations of gravity significantly increase the BR of hCMs via the LTCC.
Fichier principal
Vignette du fichier
Achyara et al_Cells_2019.pdf (1.07 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03038915 , version 1 (03-12-2020)

Identifiants

Citer

Aviseka Acharya, Sonja Brungs, Yannick Lichterfeld, Jürgen Hescheler, Ruth Hemmersbach, et al.. Parabolic, Flight-Induced, Acute Hypergravity and Microgravity Effects on the Beating Rate of Human Cardiomyocytes. Cells, 2019, 8 (4), pp.352. ⟨10.3390/cells8040352⟩. ⟨hal-03038915⟩

Collections

CNRS
11 Consultations
27 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More