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Article Dans Une Revue Genome Biology Année : 2020

FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing

Jean-Michel Arbona
Corinne Cruaud
Xia Wu
  • Fonction : Auteur
Elizaveta Novikova
  • Fonction : Auteur
Stefan Engelen
  • Fonction : Auteur
  • PersonId : 1322751
  • IdHAL : sengelen
Arnaud Lemainque
  • Fonction : Auteur
Benjamin Audit
Olivier Hyrien

Résumé

Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution. By quantifying BrdU incorporation along pulse-chased replication intermediates from Saccharomyces cerevisiae, we orient 58,651 replication tracks reproducing population-based replication directionality profiles and map 4964 and 4485 individual initiation and termination events, respectively. Although most events cluster at known origins and fork merging zones, 9% and 18% of initiation and termination events, respectively, occur at many locations previously missed. Thus, FORK-seq reveals the full extent of cell-to-cell heterogeneity in DNA replication.
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Dates et versions

hal-03055281 , version 1 (11-12-2020)

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Magali Hennion, Jean-Michel Arbona, Laurent Lacroix, Corinne Cruaud, Bertrand Theulot, et al.. FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing. Genome Biology, 2020, 21, ⟨10.1186/s13059-020-02013-3⟩. ⟨hal-03055281⟩
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