%0 Journal Article %T AP2-ERF Transcription Factors Mediate Nod Factor–Dependent Mt ENOD11 Activation in Root Hairs via a Novel cis -Regulatory Motif %+ Laboratoire des interactions plantes micro-organismes (LIPM) %+ Signalisation cellulaire et biotechnologie végétale (SCBV) %A Andriankaja, Andry %A Boisson-Dernier, Aurélien %A Frances, Lisa %A Sauviac, Laurent %A Jauneau, Alain %A Barker, David %A de Carvalho-Niebel, Fernanda %< avec comité de lecture %@ 1040-4651 %J The Plant cell %I American Society of Plant Biologists (ASPB) %V 19 %N 9 %P 2866-2885 %8 2007-10-29 %D 2007 %R 10.1105/tpc.107.052944 %M 17827349 %Z Life Sciences [q-bio]/Vegetal Biology/Botanics %Z Life Sciences [q-bio]/Genetics/Plants genetics %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]Journal articles %X Rhizobium Nod factors (NFs) are specific lipochitooligosaccharides that activate host legume signaling pathways essential for initiating the nitrogen-fixing symbiotic association. This study describes the characterization of cis-regulatory elements and trans-interacting factors that regulate NF-dependent and epidermis-specific gene transcription in Medicago truncatula. Detailed analysis of the Mt ENOD11 promoter using deletion, mutation, and gain-of-function constructs has led to the identification of an NF-responsive regulatory unit (the NF box) sufficient to direct NF-elicited expression in root hairs. NF box mediated expression requires a major GCC-like motif, which is also essential for the binding of root hair-specific nuclear factors. Yeast one-hybrid screening has identified three closely related AP2/ERF transcription factors (ERN1 to ERN3) that are able to bind specifically to the NF box. ERN1 is identical to an ERF-like factor identified recently. Expression analysis has revealed that ERN1 and ERN2 genes are upregulated in root hairs following NF treatment and that this activation requires a functional NFP gene. Transient expression assays in Nicotiana benthamiana have further shown that nucleus-targeted ERN1 and ERN2 factors activate NF box-containing reporters, whereas ERN3 represses ERN1/ERN2-dependent transcription activation. A model is proposed for the fine-tuning of NF-elicited gene transcription in root hairs involving the interplay between repressor and activator ERN factors. %G English %L hal-03082452 %U https://cnrs.hal.science/hal-03082452 %~ UNIV-TLSE3 %~ CNRS %~ ENSA-TOULOUSE %~ INRA %~ AGREENIUM %~ INRAE %~ INRAEOCCITANIETOULOUSE %~ TOULOUSE-INP %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP %~ LIPME