%0 Journal Article %T Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula %+ Laboratoire des interactions plantes micro-organismes (LIPM) %A Sauviac, Laurent %A Niebel, Andréas %A Boisson-Dernier, Aurélien %A Barker, David %A de Carvalho-Niebel, Fernanda %< avec comité de lecture %@ 0022-0957 %J Journal of Experimental Botany %I Oxford University Press (OUP) %V 56 %N 419 %P 2507-2513 %8 2005-09-01 %D 2005 %R 10.1093/jxb/eri244 %M 16043451 %K MtENOD11 %K MtENOD40 %K real time quantitative RT-PCR %K Rhizobium %K legume symbiosis %K root hair-specific expression %Z Life Sciences [q-bio]/Vegetal Biology/Botanics %Z Life Sciences [q-bio]/Genetics/Plants genetics %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]Journal articles %X This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis. After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA. This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations. Furthermore, the use of these enriched RNA samples has revealed for the first time a very significant induction (30-fold) of the MtENOD40 gene in root hairs in response to Nod factors. It is concluded that the rapid and convenient procedure described here will be particularly useful for detecting tissue-specific low-level gene expression in root hairs responding to Rhizobium Nod factors or other exogenous signals. %G English %L hal-03082465 %U https://cnrs.hal.science/hal-03082465 %~ CNRS %~ INRA %~ AGREENIUM %~ INRAE %~ INRAEOCCITANIETOULOUSE %~ LIPME