%0 Journal Article %T Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1 %+ Laboratoire des interactions plantes micro-organismes (LIPM) %A Chabaud, M. %A de Carvalho-Niebel, Fernanda %A Barker, D. %< avec comité de lecture %@ 0721-7714 %J Plant Cell Reports %I Springer Verlag %V 22 %N 1 %P 46-51 %8 2003-08 %D 2003 %R 10.1007/s00299-003-0649-y %M 12827434 %K transformation %K regeneration %K Jemalong %K AGL1 %K Agrobacterium %Z Life Sciences [q-bio]/Vegetal Biology/Botanics %Z Life Sciences [q-bio]/Genetics/Plants genetics %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X The efficiency of Agrobacterium tumefaciens transformation of the model legume Medicago truncatula cv. Jemalong (genotype 2HA) was evaluated for strains LBA 4404, C58pMP90, C58pGV2260 and AGL1. Binary vectors carrying promoter-gus/gfp reporter gene fusions and the nptII gene as selectable marker were used for plant in vitro transformation/regeneration. The highest transformation efficiency was obtained with the disarmed hypervirulent strain AGL1 (Ti plasmid TiBo542), for which the percentage of explants forming kanamycin (Km)-resistant calli was double that obtained with each of the other three strains. In addition, we were able to reduce the time necessary for plant regeneration using AGL1, with 24% of the explants generating Km-resistant transgenic plantlets within only 4-5 months of culture. Transgene expression in planta was analysed and found to be conserved in the T-1 descendents. %G English %L hal-03082480 %U https://cnrs.hal.science/hal-03082480 %~ CNRS %~ INRA %~ AGREENIUM %~ INRAE %~ INRAEOCCITANIETOULOUSE %~ LIPME %~ TEST3-HALCNRS