Differential in vitro DNA binding activity to a promoter element of the gn1 beta-1,3-glucanase gene in hypersensitively reacting tobacco plants - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Plant Journal Année : 1995

Differential in vitro DNA binding activity to a promoter element of the gn1 beta-1,3-glucanase gene in hypersensitively reacting tobacco plants

Résumé

In a hypersensitive reaction to pathogen infection, expression of the beta-1,3-glucanase gn1 gene is induced in cells surrounding the necrotic lesions. The 5'-flanking sequence of gn1 was examined to investigate the molecular basis controlling activation of gene expression during this plant defense response. Studies on transgenic tobacco plants containing gn1 promoter deletions fused to the beta-glucuronidase reporter gene revealed the presence of negative and positive regulatory sequences mediating both the level and the spatial distribution of gn1 expression. Promoter sequences to -138 bp were sufficient to confer increased gene expression around the necrotic lesions produced in response to Pseudomonas syringae pv. syringae inoculation. It is demonstrated by electrophoretic mobility shift assays that nuclear proteins in both healthy and hypersensitively reacting tobacco leaves interact with DNA sequences within the regulatory elements identified. Among the binding sequences characterized, the promoter region extending from -250 to -217 bp contained the DNA motif -GGCGGC- found to be conserved in most if not all promoters of genes encoding pathogenesis-related basic proteins. The activity bound by this promoter sequence was stronger in hypersensitively responding tissues than in healthy untreated tobacco leaves.

Dates et versions

hal-03082642 , version 1 (18-12-2020)

Identifiants

Citer

Elena Alonso, Fernanda de Carvalho Niebel, Patricia Obregon, Godelieve Gheysen, Dirk Inze, et al.. Differential in vitro DNA binding activity to a promoter element of the gn1 beta-1,3-glucanase gene in hypersensitively reacting tobacco plants. Plant Journal, 1995, 7 (2), pp.309-320. ⟨10.1046/j.1365-313x.1995.7020309.x⟩. ⟨hal-03082642⟩

Collections

CNRS
16 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More