Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
Résumé
R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner.
Domaines
Sciences du Vivant [q-bio]
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Topoisomerase I prevents transcription replication conflicts at transcription termination sites.pdf (2.74 Mo)
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Origine : Publication financée par une institution