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Poster De Conférence Année : 2014

Non-coding RNAs control of phytobeneficial genes expression by pseudomonas brassicacearum

Résumé

Rhizosphere microorganisms provide services to the plant, including growth promotion via hormone production, such as auxin, and protection against pathogens by producing some antibiotics such as cyanide and 2,4-diacetylphloroglucinol (DAPG). We recently showed that antifungal secondary metabolites (DAPG and cyanide), indoleacetate, exoenzymes (lipase and protease), and three different N-acyl-homoserine lactone molecules and other genes required for plant-bacteria interactions, are positively controlled by four non-coding RNAs rsmW, rsmX, rsmY and rsmZ. To shed light on phytobeneficial bacterial gene expression in situ, we investigated the expression of genes that positively control traits involved in phytostimulation and phytoprotection. For that purpose, we used constructs fusing the promoter of rsmW, rsmX, rsmY and rsmZ to fluorescent proteins reporter genes. To evaluate the expression level, fluorescent spectrosphotometry was used, and to image expression in planta, confocal laser microscopy was used. This study highlights the key role played by non-coding RNAs in plant-bacteria interaction.
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Dates et versions

hal-03154527 , version 1 (01-03-2021)

Identifiants

  • HAL Id : hal-03154527 , version 1

Citer

Marie Bertrand-Huleux, Sylvain Fochesato, Wafa Achouak. Non-coding RNAs control of phytobeneficial genes expression by pseudomonas brassicacearum. 11ème rencontre plantes bactéries, 2014, AUSSOIS, France. ⟨hal-03154527⟩
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