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Article Dans Une Revue Neurotherapeutics Année : 2021

Anti-prion Drugs Targeting the Protein Folding Activity of the Ribosome Reduce PABPN1 Aggregation

Rima Naït-Saïdi
  • Fonction : co premier-auteur
Martine Simonelig
Frédéric Bihel

Résumé

Prion diseases are caused by the propagation of PrP Sc , the pathological conformation of the PrP C prion protein. The molecular mechanisms underlying PrP Sc propagation are still unsolved and no therapeutic solution is currently available. We thus sought to identify new anti-prion molecules and found that flunarizine inhibited PrP Sc propagation in cell culture and significantly prolonged survival of prion-infected mice. Using an in silico therapeutic repositioning approach based on similarities with flunarizine chemical structure, we tested azelastine, duloxetine, ebastine, loperamide, metixene and showed that they all have an anti-prion activity. Like flunarizine, these marketed drugs reduced PrP Sc propagation in cell culture and in mouse cerebellum organotypic slice culture, and inhibited the protein folding activity of the ribosome (PFAR). Strikingly, some of these drugs were also able to alleviate phenotypes due to PABPN1 nuclear aggregation in cell and Drosophila models of oculopharyngeal muscular dystrophy (OPMD). These data emphasize the therapeutic potential of anti-PFAR drugs for neurodegenerative and neuromuscular proteinopathies.
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hal-03158577 , version 1 (04-03-2021)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Citer

Aline Bamia, Maha Sinane, Rima Naït-Saïdi, Jamila Dhiab, Marc Keruzoré, et al.. Anti-prion Drugs Targeting the Protein Folding Activity of the Ribosome Reduce PABPN1 Aggregation. Neurotherapeutics, 2021, 18 (2), pp.1137-1150. ⟨10.1007/s13311-020-00992-6⟩. ⟨hal-03158577⟩
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