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Article Dans Une Revue Science Advances Année : 2021

The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction

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Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V H domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V L and V H domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.
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hal-03162754 , version 1 (08-03-2021)

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Pierre Nottelet, Laure Bataille, Geraldine Gourgues, Robin Anger, Carole Lartigue, et al.. The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction. Science Advances , 2021, 7 (10), pp.eabf2403. ⟨10.1126/sciadv.abf2403⟩. ⟨hal-03162754⟩
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