A Rational Approach for the Production of Highly Soluble and Functional Sunflower Protein Hydrolysates
Résumé
Exploitation of plant proteins as an alternative to animal proteins currently presentsan important challenge for food industries. In this contribution, total sunflower protein isolatefrom cold press meal was used as a starting material for the generation of highly soluble andfunctional hydrolysates that could be used in various food formulations. To do this, a rationaland complete approach of controlled hydrolysis was implemented using the individual Alcalaseand Prolyve enzymes. The method of stopping the hydrolysis reaction was also evaluated. Theinfluence of operating conditions on hydrolysis kinetics and enzymatic mechanism was studied toidentify the appropriate hydrolysis conditions. The gain of the solubility was then analyzed andcompared to that of the initial proteins. Finally, the emulsifying and foaming properties (capacitiesand stabilities) of the resulting hydrolysates were also assessed. As a result, controlled enzymaticproteolysis significantly improved the sunflower protein solubility at neutral pH (twofold increase)and generated highly soluble hydrolysates. The limited proteolysis also maintained the good foamcapacities and allowed an improvement in the initial foam stabilities and emulsifying capacities andstabilities of sunflower proteins. This contribution can greatly increase the value of sunflower mealand help in the development of sunflower protein products in the future.
Domaines
Sciences de l'ingénieur [physics]
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