Poly(isobutylcyanoacrylate) Nanoparticles Decorated with Chitosan: Effect of Conformation of Chitosan Chains at the Surface on Complement Activation Properties
Résumé
Poly(alkylcyanoacrylate) nanoparticles decorated with chitosan were found interesting drug carriers to deliver siRNA (small interfering RNA) to tumor by intravenous injection. The nanoparticles are prepared by emulsion polymerization in presence of chitosan and pluronic® F68. The present work was aimed to study the structure of polymers composing the nanoparticles to obtain useful information about the structure of the nanoparticle surface. It was based on the characterization of nanoparticles obtained from polymerization media containing or not pluronic® F68 and cerium. It was shown that the structure of copolymers composing the nanoparticles greatly depended on the predominant mechanism of polymerization governed by the composition of the polymerization medium. Models of the conformation taken by the hydrophilic chains (chitosan and pluronic® F68) at the surface of the nanoparticles could be suggested from the structures of copolymers composing the different nanoparticles. A clear correlation of the structure of the nanoparticle surface with the capacity of the nanoparticles to trigger the activation of the complement system was established. These results will be useful to design nanoparticles with a low capacity to activate the complement system which is required to obtain stealth and safe nanoparticles.
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