Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue NPJ vaccines Année : 2021

Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine

Résumé

Mass vaccination with the live attenuated vaccine YF-17D is the current way to prevent infection with Yellow fever virus (YFV). However, 0.000012-0.00002% of vaccinated patients develop post-vaccination neurological syndrome (YEL-AND). Understanding the factors responsible for neuroinvasion, neurotropism, and neurovirulence of the vaccine is critical for improving its biosafety. The YF-FNV vaccine strain, known to be associated with a higher frequency of YEL-AND (0.3-0.4%) than YF-17D, is an excellent model to study vaccine neuroinvasiveness. We determined that neuroinvasiveness of YF-FNV occured both via infection and passage through human brain endothelial cells. Plaque purification and next generation sequencing (NGS) identified several neuroinvasive variants. Their neuroinvasiveness was not higher than that of YF-FNV. However, rebuilding the YF-FNV population diversity from a set of isolated YF-FNV-N variants restored the original neuroinvasive phenotype of YF-FNV. Therefore, we conclude that viral population diversity is a critical factor for YFV vaccine neuroinvasiveness.
Fichier principal
Vignette du fichier
s41541-021-00318-3.pdf (1.86 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-03213234 , version 1 (30-04-2021)

Licence

Paternité

Identifiants

Citer

Florian Bakoa, Christophe Préhaud, Guillaume Beauclair, Maxime Chazal, Nathalie Mantel, et al.. Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine. NPJ vaccines, 2021, 6 (1), pp.64. ⟨10.1038/s41541-021-00318-3⟩. ⟨hal-03213234⟩
64 Consultations
65 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More