High-Resolution Typing of Staphylococcus epidermidis Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Clinical Microbiology Année : 2021

High-Resolution Typing of Staphylococcus epidermidis Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones

Mathieu Coureuil
Daniel Euphrasie
  • Fonction : Auteur
  • PersonId : 913259
Julie Meyer
Jean-Philippe Barnier
Emmanuelle Bille
Alain Charbit
Xavier Nassif

Résumé

Staphylococcus epidermidis is a pathogen emerging worldwide as a leading cause of health care-associated infections. A standardized high-resolution typing method to document transmission and dissemination of multidrug-resistant S. epidermidis strains is needed. Our aim was to provide a core genome multilocus sequence typing (cgMLST) scheme for S. epidermidis to improve the international surveillance of S. epidermidis . We defined a cgMLST scheme based on 699 core genes and used it to investigate the population structure of the species and the genetic relatedness of isolates recovered from infants hospitalized in several wards of a French hospital. Our results show the long-lasting endemic persistence of S. epidermidis clones within and across wards of hospitals and demonstrate the ability of our cgMLST approach to identify and track these clones. We made the scheme publicly available through the Institut Pasteur BIGSdb server ( http://bigsdb.pasteur.fr/epidermidis/ ). This tool should enable international harmonization of the epidemiological surveillance of multidrug-resistant S. epidermidis clones. By comparing gene distribution among infection and commensal isolates, we also confirmed the association of the mecA locus with infection isolates and of the fdh gene with commensal isolates. (This study has been registered at ClinicalTrials.gov under registration no. NCT03374371.)
Fichier principal
Vignette du fichier
Jamet_Manuscript_revision3_clean.pdf (797.07 Ko) Télécharger le fichier
Figures_JCM.pdf (443.34 Ko) Télécharger le fichier
Table 1.pdf (161.61 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03218144 , version 1 (05-05-2021)

Identifiants

Citer

Anne Jamet, Julien Guglielmini, Bryan Brancotte, Mathieu Coureuil, Daniel Euphrasie, et al.. High-Resolution Typing of Staphylococcus epidermidis Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones. Journal of Clinical Microbiology, 2021, 59 (3), ⟨10.1128/JCM.02454-20⟩. ⟨hal-03218144⟩
109 Consultations
150 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More