%0 Journal Article %T Freeze-dried fecal samples are biologically active after long-lasting storage and suited to fecal microbiota transplantation in a preclinical murine model ofClostridioides difficileinfection %+ Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)) %+ Physiopathologie et Pharmacotoxicologie Placentaire Humaine (U1139) %+ AgroParisTech %+ Hôpital Beaujon [AP-HP] %A Reygner, Julie %A Charrueau, Christine %A Delannoy, Johanne %A Mayeur, Camille %A Robert, Veronique %A Cuinat, Celine %A Meylheuc, Thierry %A Mauras, Aurelie %A Augustin, Jeremy %A Nicolis, Ioannis %A Modoux, Morgane %A Joly, Francisca %A Waligora-Dupriet, Anne-Judith %A Thomas, Muriel %A Kapel, Nathalie %< avec comité de lecture %@ 1949-0976 %J Gut microbes %I Taylor & Francis %V 11 %N 5 %P 1405-1422 %8 2020 %D 2020 %R 10.1080/19490976.2020.1759489 %K CLOSTRIDIUM-DIFFICILE %K MOUSE MODEL %K ULCERATIVE-COLITIS %K INFECTION %K FROZEN %K INDUCTION %K REMISSION %K EFFICACY %Z Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X Fecal microbiota transplantation is now recommended for treating recurrent forms ofClostridioides difficileinfection. Recent studies have reported protocols using capsules of either frozen or freeze-dried stool allowing oral administration in in- and out-patient settings. However, a central question remains the viability, engraftment, and efficacy of the microbiome over time during storage life. This study shows that both the freeze-drying and freezing procedures for fecal samples allowed preserving viability, short-chain fatty acids concentration, and anti-Clostridioides difficileproperties of microbiota without significant alteration after storage for 12 months. Fecal transplantation with freeze-dried microbiota allowed engraftment of microbiota leading to clearance ofClostridioides difficileinfection in a preclinical murine model with a survival rate of 70%versus53-60% in mice treated with frozen inocula, and 20% in the untreated group. Moreover, the freeze-dried powder can be used to fill oral hard capsules using a very low amount (0.5%) of glidant excipient, allowing oral formulation. Altogether, this study showed that freeze-dried inocula can be used for the treatment ofClostridioides difficileinfection with long-lasting stability of the fecal microbiota. This formulation facilitates biobanking and allows the use of hard capsules, an essential step to simplify patient access to treatment. %G English %L hal-03292691 %U https://cnrs.hal.science/hal-03292691 %~ INSERM %~ AGROPARISTECH %~ CNRS %~ APHP %~ INC-CNRS %~ AGREENIUM %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ UP-SANTE %~ TEST-HALCNRS %~ UTCBS %~ TEST2-HALCNRS %~ ALIMH