THE VOLTAGE-GATED SODIUM CHANNEL BETA4 SUBUNIT MAINTAINS EPITHELIAL PHENOTYPE IN MAMMARY CELLS - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cells Année : 2021

THE VOLTAGE-GATED SODIUM CHANNEL BETA4 SUBUNIT MAINTAINS EPITHELIAL PHENOTYPE IN MAMMARY CELLS

Résumé

The SCN4B gene, encoding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells is not characterized. In this study, we demonstrate that reducing NaVβ4 expression in MCF10A non-cancer mammary epithelial cells generates important morphological changes observed both in two-dimensional cultures and in three-dimensional cysts. Most notably the loss of NaVβ4 induces a complete loss of epithelial organisation in cysts and increases proteolytic activity towards the extracellular matrix. Loss of epithelial morphology was associated with an increased degradation of β-catenin, reduced E-cadherin expression and induction of mesenchymal markers N-cadherin, vimentin, α-SMA expression. Overall, our results suggest that Navβ4 may participate in the maintenance of the epithelial phenotype in mammary cells and that its down regulation might be a determining step in early carcinogenesis.
Fichier principal
Vignette du fichier
CELL-REPORTS-S-21-02675.pdf (3.91 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03353576 , version 1 (24-09-2021)

Identifiants

Citer

Adélaïde Doray, Roxane Lemoine, Marc Severin, Stéphanie Chadet, Osbaldo Lopez-Charcas, et al.. THE VOLTAGE-GATED SODIUM CHANNEL BETA4 SUBUNIT MAINTAINS EPITHELIAL PHENOTYPE IN MAMMARY CELLS. Cells, 2021, ⟨10.3390/cells10071624⟩. ⟨hal-03353576⟩
55 Consultations
38 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More