%0 Journal Article %T Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells %+ Nanomédecine Régénérative (NanoRegMed) %+ Conception et application de molécules bioactives (CAMB) %+ Immuno-Rhumatologie Moléculaire %+ Laboratoire de Bioimagerie et Pathologies (LBP) %A Belcastro, Eugenia %A Rehman, Asad, Ur %A Remila, Lamia %A Park, Sin-Hee %A Gong, Dal Seong %A Anton, Nicolas %A Auger, Cyril %A Lefebvre, Olivier %A Goetz, Jacky, G %A Collot, Mayeul %A Klymchenko, Andrey, S %A Vandamme, Thierry, F %A Schini-Kerth, Valérie, B %< avec comité de lecture %@ 1549-9634 %J Nanomedicine: Nanotechnology, Biology and Medicine %I Elsevier %V 34 %8 2021-06 %D 2021 %R 10.1016/j.nano.2021.102379 %K Nanocarriers %K Endothelial senescence and dysfunction %K VCAM-1 %K Omega 3 polyunsaturated fatty acid %K UMR 1260 %K Regenerative Nanomedicine %K Faculty of Pharmacy Nanocarriers %Z Life Sciences [q-bio]/Pharmaceutical sciences/PharmacologyJournal articles %X Endothelial senescence has been identified as an early event in the development of endothelial dysfunction, a hallmark of cardiovascular disease. This study developed theranostic nanocarriers (NC) decorated with VCAM-1 antibodies (NC-VCAM-1) in order to target cell surface VCAM-1, which is overexpressed in senescent endothelial cells (ECs) for diagnostic and therapeutic purposes. Incubation of Ang IIinduced premature senescent ECs or replicative senescent ECs with NC-VCAM-1 loaded with lipophilic fluorescent dyes showed higher fluorescence signals than healthy EC, which was dependent on the NC size and VCAM-1 antibodies concentration, and not observed following masking of VCAM-1. NC loaded with omega 3 polyunsaturated fatty acid (NC-EPA:DHA6:1) were more effective than native EPA:DHA 6:1 to prevent Ang II-induced VCAM-1 and p53 upregulation, and SA-β-galactosidase activity in coronary artery segments. These theranostic NC might be of interest to evaluate the extent and localization of endothelial senescence and to prevent pro-senescent endothelial responses. %G English %2 https://cnrs.hal.science/hal-03357935/document %2 https://cnrs.hal.science/hal-03357935/file/Belcastro%20Nanomedicine%202021%20uncorrected.pdf %L hal-03357935 %U https://cnrs.hal.science/hal-03357935 %~ INSERM %~ CNRS %~ UNIV-STRASBG %~ INC-CNRS %~ SITE-ALSACE %~ TEST-HALCNRS %~ TEST2-HALCNRS