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Article Dans Une Revue Genome Biology Année : 2021

Drosophila primary microRNA-8 encodes a microRNA-encoded peptide acting in parallel of miR-8

Résumé

Background : Recent genome-wide studies of many species reveal the existence of a myriad of RNAs differing in size, coding potential and function. Among these are the long non-coding RNAs, some of them producing functional small peptides via the translation of short ORFs. It now appears that any kind of RNA presumably has a potential to encode small peptides. Accordingly, our team recently discovered that plant primary transcripts of microRNAs ( pri-miRs ) produce small regulatory peptides (miPEPs) involved in auto-regulatory feedback loops enhancing their cognate microRNA expression which in turn controls plant development. Here we investigate whether this regulatory feedback loop is present in Drosophila melanogaster.Results : We perform a survey of ribosome profiling data and reveal that many pri-miRNAs exhibit ribosome translation marks. Focusing on miR-8, we show that pri-miR-8 can produce a miPEP-8. Functional assays performed in Drosophila reveal that miPEP-8 affects development when overexpressed or knocked down. Combining genetic and molecular approaches as well as genome-wide transcriptomic analyses, we show that miR-8 expression is independent of miPEP-8 activity and that miPEP-8 acts in parallel to miR-8 to regulate the expression of hundreds of genes. Conclusion : Taken together, these results reveal that several Drosophila pri-miRs exhibit translation potential. Contrasting with the mechanism described in plants, these data shed light on the function of yet undescribed primary-microRNA -encoded peptides in Drosophila and their regulatory potential on genome expression.
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Dates et versions

hal-03360943 , version 1 (01-10-2021)

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Audrey Montigny, Patrizia Tavormina, Carine Duboe, Hélène San Clemente, Marielle Aguilar, et al.. Drosophila primary microRNA-8 encodes a microRNA-encoded peptide acting in parallel of miR-8. Genome Biology, 2021, 22, 21 p. ⟨10.1186/s13059-021-02345-8⟩. ⟨hal-03360943⟩
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