Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2021

Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection

Nicole Lieberman
Anne Moscona

Résumé

Abstract SARS-CoV-2 has caused a global pandemic of COVID-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also spread to central nervous system leading to neurological sequelae. We have developed and characterized two new organotypic cultures from hamster brainstem and lung tissues that offer a unique opportunity to study the early steps of viral infection and screening antivirals. These models are not dedicated to investigate how the virus reaches the brain. However, they allow validating the early tropism of the virus in the lungs and demonstrating that SARS-CoV-2 could infect the brainstem and the cerebellum, mainly by targeting granular neurons. Viral infection induces specific interferon and innate immune responses with patterns specific to each organ, along with cell death by apoptosis, necroptosis, and pyroptosis. Overall, our data illustrate the potential of rapid modeling of complex tissue-level interactions during infection by a newly emerged virus.
Fichier principal
Vignette du fichier
Ferren et al. Nature comm 2021.pdf (4.85 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03370276 , version 1 (15-10-2021)

Identifiants

Citer

Marion Ferren, Valérie Favède, Didier Decimo, Mathieu Iampietro, Nicole Lieberman, et al.. Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection. Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-021-26096-z⟩. ⟨hal-03370276⟩
333 Consultations
45 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More