%0 Journal Article %T LTP Induction Boosts Glutamate Spillover by Driving Withdrawal of Perisynaptic Astroglia %+ German Research Center for Neurodegenerative Diseases - Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) %+ University College of London [London] (UCL) %+ Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale (U1215 Inserm - UB) %+ The Open University [Milton Keynes] (OU) %+ University of Bonn %+ Institute of Science and Technology [Klosterneuburg, Austria] (IST Austria) %+ Australian National University (ANU) %+ Monash University [Melbourne] %+ University of Oslo (UiO) %+ Interdisciplinary Institute for Neuroscience [Bordeaux] (IINS) %A Henneberger, Christian %A Bard, Lucie %A Panatier, Aude %A Reynolds, James %A Kopach, Olga %A Medvedev, Nikolay %A Minge, Daniel %A Herde, Michel %A Anders, Stefanie %A Kraev, Igor %A Heller, Janosch %A Rama, Sylvain %A Zheng, Kaiyu %A Jensen, Thomas %A Sanchez-Romero, Inmaculada %A Jackson, Colin %A Janovjak, Harald %A Ottersen, Ole Petter %A Nagelhus, Erlend Arnulf %A Oliet, Stephane H.R. %A Stewart, Michael %A Nägerl, U. Valentin %A Rusakov, Dmitri %< avec comité de lecture %@ 0896-6273 %J Neuron %I Elsevier %V 108 %N 5 %P 919-936.e11 %8 2020-12 %D 2020 %R 10.1016/j.neuron.2020.08.030 %K Excitatory synapse %K astrocyte plasticity %K barrel cortex %K glutamate sensor imaging %K glutamate spillover %K hippocampus %K long-term potentiation %K perisynaptic astroglial processes %K super-resolution microscopy %K whisker stimulation %Z Life Sciences [q-bio]Journal articles %X Extrasynaptic actions of glutamate are limited by high-affinity transporters expressed by perisynaptic astroglial processes (PAPs): this helps maintain point-to-point transmission in excitatory circuits. Memory formation in the brain is associated with synaptic remodeling, but how this affects PAPs and therefore extrasynaptic glutamate actions is poorly understood. Here, we used advanced imaging methods, in situ and in vivo, to find that a classical synaptic memory mechanism, long-term potentiation (LTP), triggers withdrawal of PAPs from potentiated synapses. Optical glutamate sensors combined with patch-clamp and 3D molecular localization reveal that LTP induction thus prompts spatial retreat of astroglial glutamate transporters, boosting glutamate spillover and NMDA-receptor-mediated inter-synaptic cross-talk. The LTP-triggered PAP withdrawal involves NKCC1 transporters and the actin-controlling protein cofilin but does not depend on major Ca2+-dependent cascades in astrocytes. We have therefore uncovered a mechanism by which a memory trace at one synapse could alter signal handling by multiple neighboring connections. %G English %L hal-03372666 %U https://cnrs.hal.science/hal-03372666 %~ INSERM %~ CNRS %~ MAGENDIE