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Article Dans Une Revue STAR Protocols Année : 2021

Extraction and sequencing of single nuclei from murine skeletal muscles

Résumé

Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows us to sequence the myonuclei of the multinucleated myofibers. Second, it circumvents the cell-dissociation-induced transcriptional modifications. For complete details on the use and execution of this protocol, please refer to Dos Santos et al. (2020) and Machado, Geara et al. (2021).
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Dates et versions

hal-03376200 , version 1 (13-10-2021)

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Matthieu Dos Santos, Stamatia Gioftsidi, Stéphanie Backer, Léo Machado, Frederic Relaix, et al.. Extraction and sequencing of single nuclei from murine skeletal muscles. STAR Protocols, 2021, 2 (3), pp.100694. ⟨10.1016/j.xpro.2021.100694⟩. ⟨hal-03376200⟩
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