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Article Dans Une Revue Analytical Chemistry Année : 2008

Electroacoustic Polymer Microchip as an Alternative to Quartz Crystal Microbalance for Biosensor Development

Résumé

Laser photoablation of poly(ethylene terephthalate) (PET), a flexible dielectric organic polymer, was used to design an acoustic miniaturized DNA biosensor. The microchip device includes a 100-μm-thick PET layer, with two microband electrodes patterned in photoablated microchannels on one side and a depressed photoablated disk decorated by gold sputtered layer on the other side. Upon application of an electric signal between the two electrodes, an electroacoustic resonance phenomenon at ∼30 MHz was established through the microelectrodes/PET/gold layer interface. The electroacoustic resonance response was fitted with a series RLC motional arm in parallel with a static C0 arm of a Buttlerworth−Van Dyke equivalent circuit: admittance spectra recorded after successive cycles of DNA hybridization on the gold surface showed reproducible changes on R, L, and C parameters. The same hybridizations runs were performed concomitantly on a 27-MHz (9 MHz, third overtone) quartz crystal microbalance in order to validate the PET device developed for bioanalysis applications. The electroacoustic PET device, ∼100 times smaller than a microbalance quartz crystal, is interesting for the large-scale integration of acoustic sensors in biochips.

Dates et versions

hal-03406684 , version 1 (28-10-2021)

Identifiants

Citer

Jean Gamby, Mathieu Lazerges, Hubert Girault, Claude Deslouis, Claude Gabrielli, et al.. Electroacoustic Polymer Microchip as an Alternative to Quartz Crystal Microbalance for Biosensor Development. Analytical Chemistry, 2008, 80 (23), pp.8900-8907. ⟨10.1021/ac800443u⟩. ⟨hal-03406684⟩
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