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Article Dans Une Revue Organic & Biomolecular Chemistry Année : 2021

Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe

Tarushyam Mukherjee
Virupakshi Soppina
Yves Mély
Mayeul Collot
Sriram Kanvah
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Résumé

Visualization of sub-cellular organelles allows the determination of various cellular processes and the underlying mechanisms. Herein, we report a fluorescent probe, bearing push-pull substituents emitting at 600 nm and its application in cellular imaging. The probe shows dual imaging of mitochondria and nucleoli and maps mitochondrial viscosity in live cells under various physiological variations and show minimum cytotoxicity. Nucleolar staining is confirmed by RNAase digestion.

Domaines

Chimie organique
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Dates et versions

hal-03419734 , version 1 (10-11-2021)

Identifiants

Citer

Tarushyam Mukherjee, Virupakshi Soppina, Richert Ludovic, Yves Mély, Andrey S Klymchenko, et al.. Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe. Organic & Biomolecular Chemistry, 2021, 19, pp.3389 - 3395. ⟨10.1039/d0ob02378g⟩. ⟨hal-03419734⟩
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