The Autophagy Receptor TAX1BP1 (T6BP) is a novel player in antigen presentation by MHC-II molecules - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

The Autophagy Receptor TAX1BP1 (T6BP) is a novel player in antigen presentation by MHC-II molecules

Arnaud Moris
  • Fonction : Auteur
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Mathias Pereira
Clémence Richetta
  • Fonction : Auteur
Gabriela Sarango
  • Fonction : Auteur
Anita Kumari
  • Fonction : Auteur
Michael Ghosh
Lisa Bertrand
Cédric Pionneau
  • Fonction : Auteur
Morgane Le Gall
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Sylvie Grégoire
  • Fonction : Auteur
Raphaël Jeger-Madiot
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Elina Rosoy
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Mathias Faure
Audrey Esclatine
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Stéphanie Graff-Dubois
Stefan Stefanović
  • Fonction : Auteur
Bénédicte Manoury
  • Fonction : Auteur
Bertha Cecilia Ramirez
  • Fonction : Auteur

Résumé

Abstract CD4 + T lymphocytes play a major role in the establishment and maintenance of immunity. They are activated by antigenic peptides derived from extracellular or newly synthesized (endogenous) proteins presented on the surface of antigen presenting cells (APCs) by the MHC-II molecules. The pathways leading to endogenous MHC-II presentation remain poorly characterized. We demonstrate here that the autophagy receptor, T6BP, influences both autophagy-dependent and -independent endogenous presentation of HIV- and HCMV-derived peptides. By studying the immunopeptidome of MHC-II molecules, we show that T6BP affects both the quantity and quality of peptides presented. T6BP silencing induces mislocalization of the MHC-II-loading compartments and a rapid degradation of the invariant chain (CD74) without altering the expression and internalization kinetics of MHC-II molecules. We determined the interactome of T6BP in model APC and identified calnexin as a T6BP partner. Remarkably, calnexin silencing replicates the functional consequences of T6BP silencing: decreased CD4 + T cell activation and exacerbated CD74 degradation. Altogether, we unravel T6BP as a key player of the MHC-II-restricted endogenous presentation pathway and we propose one potential mechanism of action.

Dates et versions

hal-03450513 , version 1 (26-11-2021)

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Arnaud Moris, Mathias Pereira, Clémence Richetta, Gabriela Sarango, Anita Kumari, et al.. The Autophagy Receptor TAX1BP1 (T6BP) is a novel player in antigen presentation by MHC-II molecules. 2021. ⟨hal-03450513⟩
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