In vivo identification of putative CPK5 substrates in Arabidopsis thaliana - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Plant Science Année : 2022

In vivo identification of putative CPK5 substrates in Arabidopsis thaliana

Résumé

Calcium signaling mediates most developmental processes and stress responses in plants. Among plant calcium sensors, the calcium-dependent protein kinases display a unique structure harboring both calcium sensing and kinase responding activities. AtCPK5 is an essential member of this family in Arabidopsis that regulates immunity and abiotic stress tolerance. To understand the underlying molecular mechanisms, we implemented a biochemical approach to identify in vivo substrates of AtCPK5. We generated transgenic lines expressing a constitutively active form of AtCPK5 under the control of a dexamethasone-inducible promoter. Lines expressing a kinase-dead version were used as a negative control. By comparing the phosphoproteome of the kinase-active and kinase-dead lines upon dexamethasone treatment, we identified 5 phosphopeptides whose abundance increased specifically in the kinase-active lines. Importantly, we showed that all 5 proteins were phosphorylated in vitro by AtCPK5 in a calcium-dependent manner, suggesting that they are direct targets of AtCPK5. We also detected several interaction patterns between the kinase and the candidates in the cytosol, membranes or nucleus, consistent with the ubiquitous localization of AtCPK5. Finally, we further validated the two phosphosites S245 and S280 targeted by AtCPK5 in the E3 ubiquitin ligase ATL31. Altogether, those results open new perspectives to decipher AtCPK5 biological functions.
Fichier principal
Vignette du fichier
YipDelormel_MS_public.pdf (3.21 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03462313 , version 1 (01-12-2021)

Identifiants

Citer

Tiffany Yip Delormel, Liliana Avila-Ospina, Marlène Davanture, Michel Zivy, Julien Lang, et al.. In vivo identification of putative CPK5 substrates in Arabidopsis thaliana. Plant Science, 2022, 314, pp.111121. ⟨10.1016/j.plantsci.2021.111121⟩. ⟨hal-03462313⟩
193 Consultations
191 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More