Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Biomimetics Année : 2018

Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine

Résumé

Polydopamine (PDA) deposition, obtained from the oxidation of dopamine and other catecholamines, is a universal way to coat all known materials with a conformal coating which can subsequently be functionalized at will. The structural analogies between polydopamine and eumelanin, the black-brown pigment of the skin, were incited to produce stable polydopamine nanoparticles in solution, instead of amorphous precipitates obtained from the oxidation of dopamine. Herein, we demonstrate that size-controlled and colloidally stable PDA-based nanoparticles can be obtained in acidic conditions, where spontaneous auto-oxidation of dopamine is suppressed, using sodium periodate as the oxidant and a protein, like alkaline phosphatase (ALP), as a templating agent. The size of the PDA@ALP nanoparticles depends on the dopamine/enzyme ratio and the obtained particles display enzymatic activity of alkaline phosphatase, with an activity extending up to two weeks after particle synthesis. The PDA@ALP nanoparticles can be engineered in polyelectrolyte multilayered films to potentially design model biosensors. Keywords: enzymatic activity; layer-by-layer films; melanin-like nanoparticles; sodium periodate.

Domaines

Biotechnologies
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Origine : Publication financée par une institution

Dates et versions

hal-03471691 , version 1 (08-12-2021)

Identifiants

Citer

Salima El Yakhlifi, Dris Ihiawakrim, Ovidiu Ersen, Vincent Ball. Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine. Biomimetics, 2018, 3 (4), pp.36. ⟨10.3390/biomimetics3040036⟩. ⟨hal-03471691⟩
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