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Article Dans Une Revue International Journal of Pharmaceutics Année : 2020

Assessment of various formulation approaches for the application of beta-lapachone in prostate cancer therapy

Résumé

Beta-lapachone (β-Lap) is an anticancer drug activated by the NAD(P)H:quinone oxidoreductase (NQO1), an enzyme over-expressed in a large variety of tumors. B-Lap is poorly soluble in water and in most biocompatible solvents. Micellar systems, liposomes and cyclodextrins (CDs) have been proposed for its solubilization. In this work, we analyzed the properties and in vitro efficacy of β-Lap loaded in polymer nanoparticles, liposome bilayers, complexed with sulfobutyl-ether (SBE)- and hydroxypropyl (HP)-β cyclodextrins, or double loaded in phospholipid vesicles. Nanoparticles led to the lowest drug loading. Encapsulation of [β-Lap:CD] complexes in vesicles made it possible to slightly increase the encapsulation rate of the drug in liposomes, however at the cost of poor encapsulation efficiency. Cytotoxicity tests generally showed a higher sensitivity of NIH 3T3 and PNT2 cells to the treatment compared to PC-3 cells, but also a slight resistance at high β-Lap concentrations. None of the studied β-Lap delivery systems showed significant enhanced cytotoxicity against PC-3 cells compared to the free drug. Cyclodextrins and double loaded vesicles, however, appeared more efficient drug delivery systems than liposomes and nanoparticles, combining both good solubilizing and cytotoxic properties. Ligand-functionalized double loaded liposomes might allow overcoming the lack of selectivity of the drug.
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Dates et versions

hal-03489806 , version 1 (22-08-2022)

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Xiao Wu, Athena Kasselouri, Juliette Vergnaud-Gauduchon, Véronique Rosilio. Assessment of various formulation approaches for the application of beta-lapachone in prostate cancer therapy. International Journal of Pharmaceutics, 2020, 579, pp.119168. ⟨10.1016/j.ijpharm.2020.119168⟩. ⟨hal-03489806⟩
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