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Article Dans Une Revue Talanta Année : 2020

Efficient extraction of intact HSA-Aβ peptide complexes from sera: Toward albuminome biomarker identification

N. Thuy Tran
  • Fonction : Auteur correspondant
Sylvain Lehmann
Myriam Taverna

Résumé

The performances of three commercial albumin extraction methods for the isolation of intact albumin-amyloid beta peptide (HSA-Aβ) complexes from serum were compared using different analytical approaches. To determine the extraction yield, the repeatability and the selectivity of the extraction procedures, a capillary electrophoresis coupled to UV detection method was developed. For the evaluation of the specificity and integrity of the extracted HSA-Aβ complexes, SDS-PAGE, hybrid and ultra-sensitive ELISA experiments were conducted. All the extraction methods showed different characteristics depending on their chemical binding affinities toward albumin. The ProteoExtract Albumin Depletion kit extracted albumin with a high repeatability but was not efficient for the extraction of intact HSA-Aβ complexes. The PureProteome Albumin magnetic beads showed a high specificity toward HSA thanks to the grafting of anti-HSA antibodies on their surface but tended to dissociate HSA from Aβ peptides. The Pierce Albumin depletion kit showed a high extraction yield, no selectivity towards the different albumin proteoforms and proved to be the most efficient method for the extraction of intact HSA-Aβ complexes from serum.

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Chimie
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Dates et versions

hal-03490253 , version 1 (20-05-2022)

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Emilie Rossi, N. Thuy Tran, Christophe Hirtz, Sylvain Lehmann, Myriam Taverna. Efficient extraction of intact HSA-Aβ peptide complexes from sera: Toward albuminome biomarker identification. Talanta, 2020, 216, pp.121002. ⟨10.1016/j.talanta.2020.121002⟩. ⟨hal-03490253⟩
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