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Article Dans Une Revue Analytica Chimica Acta Année : 2020

Electrokinetic characterization of extracellular vesicles with capillary electrophoresis: A new tool for their identification and quantification

Résumé

This work reports on the development of the first capillary electrophoresis methodology for the elucidation of extracellular vesicles’ (EVs) electrokinetic distributions. The approach is based on capillary electrophoresis coupled with laser-induced fluorescent (LIF) detection for the identification and quantification of EVs after their isolation. Sensitive detection of these nanometric entities was possible thanks to an ‘inorganic-species-free’ background electrolyte. This electrolyte was made up of weakly charged molecules at very high concentrations to stabilize EVs, and an intra-membrane labelling approach was used to prevent EV morphology modification. The limit of detection for EVs achieved using the developed CE-LIF method reached 8 × 109 EV/mL, whereas the calibration curve was acquired from 1.22 × 1010 to 1.20 × 1011 EV/mL. The CE-LIF approach was applied to provide the electrokinetic distributions of various EVs of animal and human origins, and visualize different EV subpopulations from our recently developed high-yield EV isolation method.

Domaines

Chimie
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Dates et versions

hal-03491265 , version 1 (18-07-2022)

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Marco Morani, Thanh Duc Mai, Zuzana Krupova, Pierre Defrenaix, Evgen Multia, et al.. Electrokinetic characterization of extracellular vesicles with capillary electrophoresis: A new tool for their identification and quantification. Analytica Chimica Acta, 2020, 1128, pp.42 - 51. ⟨10.1016/j.aca.2020.06.073⟩. ⟨hal-03491265⟩
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