Metabolic profiling strategy for determination of the absorption peak of artichoke leaf extract in human enriched serum
Résumé
Our society nowadays is aging and the chronic disease appearance is increasing. To
prevent this, plant-derived nutrients like polyphenols could be used. They are known to
protect tissues from inflammatory and metabolic dysregulation induced by chronic diseases.
Polyphenols are found in high level in artichoke leaf extract (ALE) and his ingestion may be
effective to prevent such dysregulations. To prove this, an innovative clinical approach was
designed. Patients ingested ALE and their blood was collected every 20 min after the
ingestion during 240 min. The kinetic profile of the ALE absorption was studied by
1H NMR
spectroscopy. The comparison of 1D spectra showed that the highest variations were
observed in the phenolic compounds’ region and for the 100 min samples. The addition of
the degradation products of chlorogenic acid, the major ALE polyphenol compound, to a
naive serum led to similar chemical shift variations to those observed for the 100 min
samples suggesting that the metabolic changes in the serum profile after ingestion were due
to the presence of the ALE polyphenol metabolites. These results, then, allowed to continue
the clinical trial and to determine the influence of sera enriched in metabolites resulting
from ALE consumption on the behavior of human hepatocyte, adipocyte and chondrocyte
cultures. Altogether, the obtained data show that polyphenols from artichoke may represent
a relevant alternative for nutritional strategies addressing chronic disease issue [1].
References:
1. Wauquier F.; Boutin-Wittrant L.; Viret A.; Guilhaudis L et al. Nutrients. 2021, 13 (8), 2653.
DOI: https://doi.org/10.3390/nu13082653