Dynamic Membrane Localization of RNase Y in Bacillus subtilis - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue mBio Année : 2020

Dynamic Membrane Localization of RNase Y in Bacillus subtilis

Lina Hamouche
Cyrille Billaudeau
  • Fonction : Auteur
Anna Rocca
  • Fonction : Auteur
Arnaud Chastanet
Saravuth Ngo
  • Fonction : Auteur
Soumaya Laalami
  • Fonction : Auteur
Harald Putzer

Résumé

All living organisms must degrade mRNA to adapt gene expression to changing environments. In bacteria, initiation of mRNA decay generally occurs through an endonucleolytic cleavage. In the Gram-positive model organism Bacillus subtilis and probably many other bacteria, the key enzyme for this task is RNase Y, which is anchored at the inner cell membrane. While this pseudocompartmentalization appears coherent with translation occurring primarily at the cell periphery, our knowledge on the distribution and dynamics of RNase Y in living cells is very scarce. Here, we show that RNase Y moves rapidly along the membrane in the form of dynamic short-lived foci. These foci become more abundant and increase in size following transcription arrest, suggesting that they do not constitute the most active form of the nuclease. This contrasts with RNase E, the major decay-initiating RNase in E. coli , where it was shown that formation of foci is dependent on the presence of RNA substrates. We also show that a protein complex (Y-complex) known to influence the specificity of RNase Y activity in vivo is capable of shifting the assembly status of RNase Y toward fewer and smaller complexes. This highlights fundamental differences between RNase E- and RNase Y-based degradation machineries.

Dates et versions

hal-03530162 , version 1 (17-01-2022)

Identifiants

Citer

Lina Hamouche, Cyrille Billaudeau, Anna Rocca, Arnaud Chastanet, Saravuth Ngo, et al.. Dynamic Membrane Localization of RNase Y in Bacillus subtilis. mBio, 2020, 11 (1), ⟨10.1128/mBio.03337-19⟩. ⟨hal-03530162⟩
14 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More