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Article Dans Une Revue Analytical Chemistry Année : 2022

Single-Molecule Sandwich Aptasensing on Nanoarrays by Tethered Particle Motion Analysis

Résumé

High-throughput single-molecule techniques are expected to challenge the demand of rapid, simple and sensitive detection methods in the health and environmental fields. Based on a single-DNA molecule biochip for the parallelization of Tethered Particle Motion analyses by videomicroscopy coupled to image analysis and its smart combination with aptamers, we successfully developed an aptasensor enabling the detection of single target molecules by a sandwich assay. One aptamer is grafted to the nanoparticles tethered to the surface by a long DNA molecule bearing the second aptamer in its middle. The detection and quantification of the target is direct. The recognition of the target by the pair of aptamers leads to a looped configuration of the DNA-particle complex associated to a restricted motion of the particles, which is monitored in real-time. An analytical range extending over three decades of target concentration with a limit of detection in the picomolar range was obtained for thrombin.

Domaines

Chimie Biophysique
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Dates et versions

hal-03622831 , version 1 (30-01-2023)

Identifiants

Citer

Diana Soukarié, Philippe Rousseau, Maya Salhi, Alexia de Caro, Jean-Marc Escudier, et al.. Single-Molecule Sandwich Aptasensing on Nanoarrays by Tethered Particle Motion Analysis. Analytical Chemistry, 2022, 94 (10), pp.4319-4327. ⟨10.1021/acs.analchem.1c04995⟩. ⟨hal-03622831⟩
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