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Article Dans Une Revue Methods in Molecular Biology Année : 2017

Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C

Résumé

The chromosome conformation capture (3C) method has been invaluable in studying chromatin interactions in a population of cells at a resolution surpassing that of light microscopy, for example in the detection of functional contacts between enhancers and promoters. Recent developments in sequencing-based chromosomal contact mapping (Hi-C, 5C and 4C-Seq) have allowed researchers to interrogate pairwise chromatin interactions on a wider scale, shedding light on the three-dimensional organization of chromosomes. These methods present significant technical and bioinformatic challenges to consider at the start of the project. Here, we describe two alternative methods for Hi-C, depending on the size of the genome, and discuss the major computational approaches to convert the raw sequencing data into meaningful models of how genomes are organized.
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Dates et versions

hal-03676440 , version 1 (23-05-2022)

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Matteo Viettri Rudan, Suzana Hadjur, Thomas Sexton. Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C. Methods in Molecular Biology, 2017, 1589, pp.47-74. ⟨10.1007/7651_2015_261⟩. ⟨hal-03676440⟩
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