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Article Dans Une Revue Matrix Biology Année : 2017

Integrin alpha6 maintains the structural integrity of the kidney collecting system

Résumé

Laminins are a major constituent of the basement membranes of the kidney collecting system. Integrins, transmembrane receptors formed by non-covalently bound alpha and beta subunits, serve as laminin receptors, but their role in development and homeostasis of the kidney collecting system is poorly defined. Integrin alpha3beta1, one of the major laminin receptors, plays a minor role in kidney collecting system development, while the role of alpha6 containing integrins (alpha6beta1 and alpha6beta4), the other major laminin receptors, is unknown. Patients with mutations in alpha6 containing integrins not only develop epidermolysis bullosa, but also have abnormalities in the kidney collecting system. In this study, we show that selectively deleting the alpha6 or beta4 integrin subunits at the initiation of ureteric bud development in mice does not affect morphogenesis. However, the collecting system becomes dilated and dysmorphic as the mice age. The collecting system in both null genotypes was also highly susceptible to unilateral ureteric obstruction injury with evidence of excessive tubule dilatation and epithelial cell apoptosis. Mechanistically, integrin alpha6-null collecting duct cells are unable to withstand high mechanical force when adhered to laminin. Thus, we conclude that alpha6 integrins are important for maintaining the integrity of the kidney collecting system by enhancing tight adhesion of the epithelial cells to the basement membrane. These data give a mechanistic explanation for the association between kidney collecting system abnormalities in patients and epidermolysis bullosa.

Dates et versions

hal-03691194 , version 1 (08-06-2022)

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Citer

Olga Viquez, Eugenia Yazlovitskaya, Tianxiang Tu, Glenda Mernaugh, Pablo Secades, et al.. Integrin alpha6 maintains the structural integrity of the kidney collecting system. Matrix Biology, 2017, 57-58, pp.244-257. ⟨10.1016/j.matbio.2016.12.003⟩. ⟨hal-03691194⟩
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