Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue European Journal of Immunology Année : 2021

Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections

Matthew Hockin
  • Fonction : Auteur
Andrew Hemmert
  • Fonction : Auteur

Résumé

Low concentrations of type-I interferon (IFN) in blood seem to be associated with more severe forms of Coronavirus disease 2019 (COVID-19). However, following the type-I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature on a FilmArray® system, which includes PCR assays for four interferon stimulated genes. We analyzed three types of patient populations: (i) children admitted to a pediatric emergency unit for fever and suspected infection, (ii) ICU-admitted patients with severe COVID-19, and (iii) healthcare workers with mild COVID-19. The results were compared to the reference tools, that is, molecular signature assessed with Nanostring® and IFN-α2 quantification by SIMOA® (Single MOlecule Array). A strong correlation was observed between the IR measured by the FilmArray®, Nanostring®, and SIMOA® platforms (r-Spearman 0.996 and 0.838, respectively). The FilmArray® panel could be used in the COVID-19 pandemic to evaluate the IR in 45-min with 2 min hand-on-time at hospitalization and to monitor the IR in future clinical trials.

Dates et versions

hal-03708117 , version 1 (29-06-2022)

Identifiants

Citer

Marine Mommert, Magali Perret, Matthew Hockin, Sébastien Viel, Alexandre Belot, et al.. Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections. European Journal of Immunology, 2021, 51 (4), pp.989-994. ⟨10.1002/eji.202048978⟩. ⟨hal-03708117⟩
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