DNA demethylation and hypermethylation are both required for late nodule development in Medicago - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nature Plants Année : 2022

DNA demethylation and hypermethylation are both required for late nodule development in Medicago

Résumé

Plant epigenetic regulations are involved in transposable element silencing, developmental processes and responses to the environment1,2,3,4,5,6,7. They often involve modifications of DNA methylation, particularly through the DEMETER (DME) demethylase family and RNA-dependent DNA methylation (RdDM)8. Root nodules host rhizobia that can fix atmospheric nitrogen for the plant's benefit in nitrogen-poor soils. The development of indeterminate nodules, as in Medicago truncatula, involves successive waves of gene activation9,10,11,12, control of which raises interesting questions. Using laser capture microdissection (LCM) coupled to RNA-sequencing (SYMbiMICS data11), we previously identified 4,309 genes (termed NDD) activated in the nodule differentiation and nitrogen fixation zones, 36% of which belong to co-regulated genomic regions dubbed symbiotic islands13. We found MtDME to be upregulated in the differentiation zone and required for nodule development, and we identified 474 differentially methylated regions hypomethylated in the nodule by analysing ~2% of the genome4. Here, we coupled LCM and whole-genome bisulfite sequencing for a comprehensive view of DNA methylation, integrated with gene expression at the tissue level. Furthermore, using CRISPR–Cas9 mutagenesis of MtDRM2, we showed the importance of RdDM for CHH hypermethylation and nodule development. We thus proposed a model of DNA methylation dynamics during nodule development.
Fichier principal
Vignette du fichier
Pecrix-et-al_NaturePlants_2022_HAL.pdf (5.13 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03728475 , version 1 (20-07-2022)

Identifiants

Citer

Yann Pecrix, Erika Sallet, Sandra Moreau, Olivier Bouchez, Sébastien Carrere, et al.. DNA demethylation and hypermethylation are both required for late nodule development in Medicago. Nature Plants, 2022, 8 (7), pp.741-749. ⟨10.1038/s41477-022-01188-w⟩. ⟨hal-03728475⟩
51 Consultations
148 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More