%0 Journal Article %T Memory CD4+ T-Cell Lymphocytic Angiopathy in Fatal Forms of COVID-19 Pulmonary Infection %+ Centre d'Immunologie et des Maladies Infectieuses (CIMI) %+ CHU Pitié-Salpêtrière [AP-HP] %+ Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP) %+ Lymphocytes B, Autoimmunité et Immunothérapies (LBAI) %+ Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN) %A Guihot, Amélie %A Plu, Isabelle %A Soulié, Cathia %A Rousseau, Alice %A Nakid-Cordero, Cecilia %A Dorgham, Karim %A Parizot, Christophe %A Litvinova, Elena %A Mayaux, Julien %A Malet, Isabelle %A Quentric, Paul %A Combadière, Béhazine %A Combadière, Christophe %A Bonduelle, Olivia %A Adam, Lucille %A Rosenbaum, Pierre %A Beurton, Alexandra %A Hémon, Patrice %A Debré, Patrice %A Vieillard, Vincent %A Autran, Brigitte %A Seilhean, Danielle %A Charlotte, Frédéric %A Marcelin, Anne-Geneviève %A Gorochov, Guy %A Luyt, Charles-Edouard %< avec comité de lecture %@ 1664-3224 %J Frontiers in Immunology %I Frontiers %V 13 %P 844727 %8 2022-04-22 %D 2022 %R 10.3389/fimmu.2022.844727 %M 35529881 %K Autopsia %K Broncho-alveolar lavage (BAL) %K COVID-19 %K T cell responses %K Vasculitis %Z Life Sciences [q-bio]Journal articles %X The immunopathological pulmonary mechanisms leading to Coronavirus Disease (COVID-19)-related death in adults remain poorly understood. Bronchoalveolar lavage (BAL) and peripheral blood sampling were performed in 74 steroid and non-steroid-treated intensive care unit (ICU) patients (23–75 years; 44 survivors). Peripheral effector SARS-CoV-2-specific T cells were detected in 34/58 cases, mainly directed against the S1 portion of the spike protein. The BAL lymphocytosis consisted of T cells, while the mean CD4/CD8 ratio was 1.80 in non-steroid- treated patients and 1.14 in steroid-treated patients. Moreover, strong BAL SARS-CoV-2 specific T-cell responses were detected in 4/4 surviving and 3/3 non-surviving patients. Serum IFN-γ and IL-6 levels were decreased in steroid-treated patients when compared to non-steroid treated patients. In the lung samples from 3 (1 non-ICU and 2 ICU) additional deceased cases, a lymphocytic memory CD4 T-cell angiopathy colocalizing with SARS-CoV-2 was also observed. Taken together, these data show that disease severity occurs despite strong antiviral CD4 T cell-specific responses migrating to the lung, which could suggest a pathogenic role for perivascular memory CD4 T cells upon fatal COVID-19 pneumonia. %G English %L hal-03745734 %U https://cnrs.hal.science/hal-03745734 %~ INSERM %~ UNIV-BREST %~ CNRS %~ APHP %~ CIMI %~ IPLESP %~ ICAN %~ IMMUNO %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ IBSAM %~ SU-MEDECINE %~ SU-MED %~ SU-TI %~ ALLIANCE-SU %~ NANTES-UNIVERSITE %~ NANTES-UNIV %~ IHU_ICAN