%0 Journal Article %T Generation of an Adequate Perfusion Network within Dense Collagen Hydrogels Using Thermoplastic Polymers as Sacrificial Matrix to Promote Cell Viability %+ Matériaux et Biologie (LCMCP-MATBIO) %+ Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A) %A Camman, Marie %A Marquaille, Pierre %A Joanne, Pierre %A Agbulut, Onnik %A Hélary, Christophe %< avec comité de lecture %@ 2306-5354 %J Bioengineering %I MDPI %V 9 %8 2022-07-14 %D 2022 %R 10.3390/bioengineering9070313 %K thermoplastic polymers %K HIPS %K sacrificial matrix %K perfusion %K dense collagen hydrogel %Z Chemical Sciences/Material chemistryJournal articles %X TDense collagen hydrogels are promising biomaterials for several tissue-engineering applications.They exhibit high mechanical properties, similar to physiological extracellular matrices,and do not shrink under cellular activity. However, they suffer from several drawbacks, such asweak nutrient and O2 diffusion, impacting cell survival. Here, we report a novel strategy to createa perfusion system within dense and thick collagen hydrogels to promote cell viability. The 3Dprinting of a thermoplastic filament (high-impact polystyrene, HIPS) with a three-wave shape is usedto produce an appropriate sacrificial matrix. The HIPS thermoplastic polymer allows for good shapefidelity of the filament and does not collapse under the mechanical load of the collagen solution. Afterthe collagen gels around the filament and dissolves, a channel is generated, allowing for adequateand rapid hydrogel perfusion. The dissolution process does not alter the collagen hydrogel’s physicalor chemical properties, and the perfusion is associated with an increased fibroblast survival. Here,we report the novel utilization of thermoplastics to generate a perfusion network within biomimeticcollagen hydrogels. %G English %2 https://hal.sorbonne-universite.fr/hal-03780021/document %2 https://hal.sorbonne-universite.fr/hal-03780021/file/bioengineering-09-00313-1.pdf %L hal-03780021 %U https://hal.sorbonne-universite.fr/hal-03780021 %~ INSERM %~ CNRS %~ LCMCP %~ B2A %~ INC-CNRS %~ LCMCP-MATBIO %~ IBPS %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ SU-SCIENCES %~ SU-TI %~ ALLIANCE-SU %~ TEST2-HALCNRS %~ CHIMIE-SU