%0 Unpublished work %T Escape From Oncogene-Induced Senescence is Controlled by POU2F2 and Memorized by Chromatin Scars %+ Rutgers University [Newark] %+ Rutgers, The State University of New Jersey [New Brunswick] (RU) %+ Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A) %+ Biologie des ARN de Plasmodium - Plasmodium RNA Biology %+ Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM) %+ Rutgers New Jersey Medical School (NJMS) %+ Centre National de la Recherche Scientifique (CNRS) %A Martínez-Zamudio, Ricardo Iván %A Stefa, Alketa %A Nabuco, José Américo %A Vasilopoulos, Themistoklis %A Simpson, Mark %A Doré, Gregory %A Roux, Pierre-François %A Galan, Mark %A Chokshi, Ravi %A Bischof, Oliver %A Herbig, Utz %Z This study was supported by the National Cancer Institute of the NIH (R01CA136533). %Z Posté sur BIORXIV le 2 juillet 2022 %8 2022-10-05 %D 2022 %R 10.1101/2022.06.29.497942 %Z Life Sciences [q-bio] %Z Life Sciences [q-bio]/Cellular BiologyPreprints, Working Papers, ... %X Although oncogene-induced senescence (OIS) is a potent tumor-suppressor mechanism, recent studies revealed that cells can escape from OIS with features of transformed cells. However, the mechanisms that promote OIS escape remain unclear, and evidence of post-senescent cells in human cancers is missing. Here, we unravel the regulatory mechanisms underlying OIS escape using dynamic multidimensional profiling. We demonstrate a critical role for AP1 and POU2F2 transcription factors for escape from OIS and identify ‘senescence-associated chromatin scars (SACS)’ as an epigenetic memory of OIS, detectable during colorectal cancer progression. POU2F2 levels are elevated already in precancerous lesions and as cells escape from OIS, and its expression and binding activity to cis-regulatory elements are associated with decreased patient survival. Our results support a model in which POU2F2 exploits a precoded enhancer landscape to promote senescence escape and reveal POU2F2 gene signatures and SACS as valuable biomarkers with diagnostic and prognostic potential. %G English %2 https://hal.science/hal-03799233/document %2 https://hal.science/hal-03799233/file/2022.06.29.497942v1.full.pdf %L hal-03799233 %U https://hal.science/hal-03799233 %~ INSERM %~ CNRS %~ FNCLCC %~ VALDAURELLE %~ B2A %~ IBPS %~ UNIV-MONTPELLIER %~ IRCM %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ SU-SCIENCES %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ SU-TI %~ ALLIANCE-SU %~ UM-2015-2021 %~ UM-EPE