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Article Dans Une Revue Journal of Cell Biology Année : 2022

Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole

Jierui Zhao
Mai Thu Bui
  • Fonction : Auteur
Juncai Ma
Fabian Künzl
  • Fonction : Auteur
Lorenzo Picchianti
  • Fonction : Auteur
Yixuan Chen
  • Fonction : Auteur
Sofia Petsangouraki
Azadeh Mohseni
Marta Salas Gomez
Caterina Giannini
  • Fonction : Auteur
Dubois Gwennogan
Roksolana Kobylinska
  • Fonction : Auteur
Swen Schellmann
  • Fonction : Auteur
Yvon Jaillais
Byung-Ho Kang
  • Fonction : Auteur
  • PersonId : 1113129

Résumé

Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also localizes to the autophagosomes and plays a role in autophagic flux in the liverwort Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes with the multivesicular body-localized ESCRT-I component VPS23A, leading to the formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation. Altogether, our results reveal a conserved vacuolar sorting hub that regulates autophagic flux in plants.
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hal-03823604 , version 1 (21-10-2022)

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Jierui Zhao, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti, et al.. Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. Journal of Cell Biology, 2022, 221 (12), pp.e202203139. ⟨10.1083/jcb.202203139⟩. ⟨hal-03823604⟩
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