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Article Dans Une Revue Surfaces and Interfaces Année : 2022

Surface functionalization strategy to enhance the antibacterial effect of nisin Z peptide

Stratégie de fonctionnalisation de surface pour amplifier le caractère antibactérien du peptide nisin Z.

Résumé

One of the main challenges when building antibacterial surfaces with antimicrobial peptides (AMPs) is to preserve their antimicrobial activity after stable immobilization of the peptides. Among all parameters, order/conformation of self-assembled monolayers, used as spacer, is one the most important. Herein we report the covalent immobilization of the nisin Z peptide on a gold surface functionalized with a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) alone or mixed with 6-mercaptohexanol, used as a spacer. The MUA acid is activated by treatment with carbodiimide/N-hydroxysuccinimidine and then reacts with nisin Z to form amide bonds via the N terminal part of the peptide. We have characterized each step of the surface modification using X-ray photoelectron spectroscopy, FTIR-ATR spectroscopy and contact angle measurements. The combined results show the success of each functionalization step. Additionally, SFG brings information on the orientation and conformational ordering of the self-assembled monolayers. Indeed, a better order of MUA25 layers compared to MUA was observed due to the spacing of carboxylic acid groups. The antibacterial activity of the immobilized AMPs against Staphylococcus aureus is evaluated using confocal microscopy and bacterial counting: it increases with a better order of the SAMs rather than a greater peptide concentration. This study provides fundamental insights on how to engineer AMPs and substrate to produce efficient biocidal surfaces.
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Licence : Domaine public
Commentaire : Supplementary information

Dates et versions

hal-03838980 , version 1 (16-11-2022)

Identifiants

Citer

Pascal Thébault, Mohamad Ammoun, Rym Boudjemaa, Aimeric Ouvrard, Karine Steenkeste, et al.. Surface functionalization strategy to enhance the antibacterial effect of nisin Z peptide. Surfaces and Interfaces, 2022, 30 (101822), pp.1-8. ⟨10.1016/j.surfin.2022.101822⟩. ⟨hal-03838980⟩
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